Our new Obvious+ tissue clearing procedure is the only real method that delipidates samples without any modify in morphology and with small influence on structural integrity.
Megatome is a vibrating microtome made to section a wide selection of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With large blade vibrating frequency and minimized blade deflection, Megatome allows substantial-throughput tissue sectioning with uniform floor profile, and minimal tissue hurt and data reduction.
Antibodies may perhaps take months to diffuse as a result of only some millimeters of tissue, with a steep labeling gradient from surface to Main.
Megatome is suitable for precision: the blade vibrates at the next frequency and larger amplitude array than other microtomes, and includes a distinctive deflection Manage mechanism.
SE employs a rotational electrical subject to disperse extremely electromobile molecules (like antibodies or surfactant micelles) all through a porous sample without harming electrically billed constructions inside the tissue. This enables two-four working day clearing of intact organs,
Our preformulated EasyIndex Option raises and homogenize the refractive index of delipidated tissue samples, rendering them thoroughly transparent. This enables gentle penetration to the sample and guarantees the acquisition of substantial-resolution, in-target image facts.
Megatome can be a novel microtome that allows for top-precision sectioning of an array of tissue samples – from organoids, to arrays of animal organs, to intact human Mind hemispheres – with nominal tissue damage and knowledge loss.
It’s time to update your microtome to Megatome. With exact large-frequency slicing for an unmatched selection of sample sizes and kinds – from organoids and tumors to expanded tissues, sample arrays, and intact primate organs – Megatome is optimized for numerous apps.
The SmartSPIM mild sheet microscope supplies unparalleled resolution and acquisition velocity all over intact tissue volumes, while its modular design and style means that you can up grade and customise specific factors.
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Possibly down load a release or create a distribution zip as outlined higher than. Unzip the archive to your sought after area.
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Our novel SHIELD tissue preservation method kinds intramolecular bonds employing polyfunctional, adaptable epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids.